ahpA [2019-08-01 08:28:09]

alkyl hydroperoxide reductase

locus: BSU_14220

pI: 4.49

mw: 20.30 kDa

protein length: 180 aa

gene length: 543 bp

function: protection against peroxide stress

product: alkyl hydroperoxide reductase

essential: no

synonyms: ykuU

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]

Gene

Coordinates: 1,492,261 → 1,492,803

The protein

Protein family

• [SW|peroxiredoxin family] (according to UniProt)

Paralogous protein(s)

• [protein|D0982500E52577D52FADF775C0E512A4B9657B79|AhpC]

Structure

• [PDB|4XCS] (human peroxiredoxin, 48% identity)

Expression and Regulation

Operons

• genes: [gene|B283B702D917E310130BC33A40BF6A5853C3D4C1|ahpA]-[gene|398E6FAC317D9BD81FB80A7CC9DF1CC757F2FA98|ahpT]
  description: [Pubmed|20817675]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

Biological materials

Mutant

• GP1729 [gene|search|ahpAT]::kan trpC2 available at Jörg Stülkes lab
• MGNA-B343 (ykuU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1342 NBRP B. subtilis, Japan]
• BKE14220 (Δ[gene|B283B702D917E310130BC33A40BF6A5853C3D4C1|ahpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCCCTCCAATTT, downstream forward: _UP4_TAATTCTTTCCAAGAACGAA
• BKK14220 (Δ[gene|B283B702D917E310130BC33A40BF6A5853C3D4C1|ahpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14220 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCCCTCCAATTT, downstream forward: _UP4_TAATTCTTTCCAAGAACGAA

References

• 20817675,26787766,27683249